The Center for Computational and Integrative Biology (CCIB) Confocal Imaging Core Facility is located at the Joint Health Sciences Center in Camden, NJ. The facility is organized to encourage the use of the equipment by individual users both within and outside the university. Individual and small-group instruction in the use of equipment and in confocal microscopy techniques is provided to faculty, staff, post-doctoral fellows and residents for a set fee.
Available Equipment
Inverted Stand DMI 6000 with Adaptive Focus Control (AFC)
Objectives-10X/0.30, 63X/1.40 oil
Filter Cubes- DAPI, CFP, GFP, Rhodamine, DIC
Confocal Tandem Scanner – FOV scanner and 8kHz resonance scanner
Excitation Laser Lines: 405, 458, 488, 514, 561, 633 nm
Emission Tunable Spectral Detectors:
- 2 PMT
- 2 HyD
- 1 HyD2-SMD
SuperZ galvo for xz scanning
Fluorescence Correlation Spectroscopy (FCS)
Premium CUDA workstation
Leica LAS-X imaging software package
Sample Format- slides, glass-bottom dish, microwell plate
Recent Upgrade to Leica’s LIGHTNING imaging software
LIGHTNING is a new method for fully automated intelligent information extraction from confocal data in near real time using extremely fast parallel GPU processing. Confocal images acquired with LIGHTNING appear as if a layer of noise has been removed from them compared to what has been the standard so far. The resulting images reveal a clear and unobstructed view of the relevant information.
Inverted Stand DMI 6000 with Adaptive Focus Control (AFC)
Objectives-10X/0.30, 20X/0.75, 40X/1.3 oil, 100X/1.44 oil
Filter Cubes- DAPI, GFP, Rhodamine, DIC
Confocal FOV Scanner
Excitation Laser Lines: 405, 488, 552, 638 nm
Emission Tunable Spectral Detectors:
- 2 PMT
- 2 HyD
SuperZ galvo for xz scanning
Leica LAS-X software package
Sample Format- slides, glass-bottom dish, microwell plate
Microscope usage is scheduled through iLab
Information on Rutgers Core Facilities