The CCIB seminar series offers interesting presentations related to research in computational and integrative biology. The presentations reflect the types of collaborative research being done at the center and provide an opportunity to come together as a community for informal discussions and conversation.
Unless noted, all seminars take place during the free period (12:20pm – 1:20pm) in the Business and Science Building, Room 132.
Coffee and snacks are provided.
Tuesday, October 20, 2015
Dr. Jia Song, University of Delaware
TITLE: microRNA cross-regulation of signaling pathways
ABSTRACT: In early development, cell specification and pattern formation are controlled by cross-regulation of gene regulatory networks (GRN) and signaling pathways. Signaling morphogen gradients are critical regulators that need to be tightly controlled in order to ensure that the precise organization of the embryo is achieved. This research addresses the overarching hypothesis that microRNAs (miRNAs) perform this critical regulatory function. The miRNAs are small non-coding RNAs that repress the translation and reduce the stability of target mRNAs in animal cells. microRNA-31 (miR-31) has been found to play a role in cancer, bone formation, and lymphatic development. However, the function of miR-31 in embryogenesis is not known. We examined the regulatory role of miR-31 in early development of the sea urchin. We found that miR-31 is expressed in all stages of development and its knockdown (KD) results in decrease in embryo size, formation of extra cells and cell clumps in the blastocoelar space of the embryo and disrupts the patterning and function of the primary mesenchyme cells (PMCs), which form the embryonic skeleton spicules. Using bioinformatics approach and luciferase reporter constructs, we identified miR-31 to repress directly Pmar1, Alx1, Snail and VegfR7 within the PMC GRN. Further, blocking the miR-31-mediated repression of Alx1 and/or VegfR7 genes in the developing embryo resulted in defects in PMC patterning and skeletogenesis. The majority of the mislocalized PMCs in miR-31 KD embryos did not express VegfR10, indicating that miR-31 regulated VegfRs within the PMCs. In addition, miR-31 indirectly suppresses Vegf3 expression in the ectoderm. These results indicate that miR-31 coordinately suppresses genes within the PMCs and in the ectoderm to impact PMC patterning and skeletogenesis.
Fall 2015 Seminar Speakers
- September 15, 2015: Dr. Feng Gai; University of Pennsylvania
- September 22, 2015: Nicole Pope; CCIB Student
- September 29, 2015: Daniel Russo; CCIB Student
- October 6. 2015: Wenyi Wang; CCIB Student
- October 13, 2015: Sean Ekins; CSO of Collaborative Drug Discovery
- October 20, 2015: Jia Song; University of Delaware
- October 27, 2015: Lewis-Burke Associates – YOU MUST RSVP BY 10/20 to Caryn Terry
- November 3, 2015: Sweta Sharma; CCIB Student
- November 10, 2015: Yinghui Zhong; Drexel University
- November 17, 2015: Marc Halfon; SUNY at Buffalo
- November 24, 2015: Tianhai Tian; Monash University
- December 1, 2015: Dr. Robert Best, NIH
Visit http://ccib.camden.rutgers.edu/seminars/past-seminars/ for a list of all past CCIB seminars.
CCIB Seminar Evaluation Form:
If you are a registered student for CCIB Seminar, you will need to complete a seminar evaluation form after each seminar.